"Genetic modification is a success!"
Seeing the pictures of cells under the microscope, Huang Qin breathed a long sigh of relief, and his hanging heart finally relaxed.
A burst of cheers suddenly broke out behind him.
"Yeah, I just said it's okay."
"Is this it?"
"That's great, long live Senior Brother."
"Don't be too happy yet, this is just the first step."
"Yes, the next step of cloning the embryo is the key."
The students were all chattering away, unable to conceal their excitement.
Then, they all turned their heads and looked at their beloved senior brother expectantly.
"When will the next step begin?"
Huang Qin looked at the expectant eyes of his junior classmates and couldn't help but laugh.
I remember when I forced them to do experiments every day, these rookies all showed resistance and looked like they were worse off than dead.
If it weren't for the carrot of graduation hanging in front of them, he would have doubted whether these people could persevere.
I didn’t expect it, but now I’m gearing up and can’t wait.
And in turn, he urged himself.
This situation reminded him of the time when his first experiment was successful and his first project achieved results.
I am so excited that I wish I could spend 28 hours a day in the laboratory, witnessing the miracles of nature one after another.
Thinking of this, Huang Qin couldn't help but raise the corners of his mouth, and comforted him with a smile.
"I know you are in a hurry, but don't be in a hurry, we'll start right away."
"You have mastered the basic process of cloning mice through nuclear transfer."
The classmates nodded one after another, as obediently as chickens pecking at rice.
"Okay, I will give a demonstration first, and then you will divide the cells into parts to make pig nuclear transfer clones."
"When the time comes I want to see whose embryos do the best."
The students suddenly burst into laughter.
"Oh, Senior Brother, I have cloned mice a hundred and eighty times. I am absolutely familiar with it and I will not let you down."
"Okay, look at me! Let me show you what it means to be better than the old."
"If you do a good job, is there any reward?"
Huang Qin touched his head and smiled.
"Hey, you still want to be better than the old? It seems that I underestimated you."
"Okay, as long as everyone's embryos can survive for more than 20 days, I will take you to team building for two days. You can choose the location. How about it?"
Generally speaking, in the process of cloning animals, there are three peak periods of embryonic death.
Around 10 days is the first peak period. In the first 5 days, the pig egg cell will divide into 70-100 cells, forming a structure called a blastocyst.
By about 10 days, the blastocyst will hatch into an embryo, ready for implantation in the uterus.
During this period, the environment of the uterus must be simulated in vitro. A slight error can easily cause embryonic death, with a mortality rate of up to 20%.
Around 20 days is the second peak period. The embryo has implanted, but the organs have not yet formed. They are all competing for nutrients secreted by the placenta. Some embryos with weaker abilities will fail in the competition and die one after another.
Embryonic mortality at this stage is as high as 15%.
In about 60 days, we will usher in the third peak period.
However, this stage is not bad. It can be avoided by providing adequate nutrition and the mortality rate will be greatly reduced.
Therefore, in the entire cloning process, the first 20 days are very critical.
Not only must they develop into embryos outside the body, but they must also be successfully implanted into the sow and survive a period of competition with each other in order to survive.
This is why Huang Qin proposed the assessment.
After hearing this, the students all looked embarrassed. After all, pig embryos are more complicated than mice. Just because mice can be successfully cloned does not mean that pigs can be successful.
Besides, this is a batch of genetically modified pigs. Who knows what the consequences of a genetic modification will be.
It is very likely that the entire army will be wiped out and no one will survive.
The tall and lanky boy gritted his teeth and said loudly: "No problem, I have confidence in myself."
Huang Qin immediately cast an admiring glance. He had noticed this tall and thin boy for a long time. He was excellent in all aspects and was a good prospect for scientific research.
Seeing that someone stood out, others also joined in.
"Look at me, Senior Brother, I will definitely give you a big surprise."
"For these two days of team building, I will work hard no matter what!"
"Wow, it happens to be winter now. I want to go to the warm seaside and bask in the sun."
"If you don't go to the beach, it would be nice to find a hot spring hotel, soak in the hot springs for two days, and eat and drink."
Huang Qin nodded with satisfaction. Since the thesis was basically complete, the pressure of graduation dropped drastically.
For the next difficult work, he could only rely on money to motivate everyone. Now it seems that the effect is very good.
Students have stayed in the ivory tower for a long time and have not yet seen the horror of sugar-coated bullets in the outside world, so they are easily bribed.
"College students are so easy to fool! If it were the veterans in our department, they wouldn't be fooled so easily."
He couldn't help but sigh in his heart: "It's really useful for college students. It would be great if the teacher could take care of a few more students."
The rest will be easy.
Huang Qin gave everyone a demonstration first.
The sows used in the laboratory are very different from ordinary domestic pigs. They are specially optimized mini pigs.
It is about the same size as a dog, and even when mature it only weighs 15 kilograms.
The piglets used in the experiment are all 3-5 months old. They weigh only a few kilograms like cats and are exceptionally small and exquisite.
They have fluffy white body hair, black flake patterns on their backs, pink noses and mouths, and rolling eyes, which are very cute.
No matter how cute he is, he still has to devote himself to human technology.
"Senior brother, let me catch the second senior brother, hey hey hey!"
The female classmate with a high ponytail volunteered to stand up, beaming with joy.
She grabbed a gilt from the cage. The pig had been injected with chorionic gonadotropin several times to induce a state of high egg production.
Looking at the pink and cute piglet on her hand, the female classmate looked unbearable, but her movements were unambiguous.
He raised the needle and put a tube of anesthetic in, and the little sow instantly fell into a coma.
Fresh oocytes were then collected from the sow's oviduct.
Under a microscope, Huang Qin carefully used a thin tube with a diameter of about 7 microns to remove the nucleus of the oocyte and removed as much cytoplasm as possible.
While doing it, he explained the precautions to his classmates, and he also made a few jokes.
"After taking out the cell nucleus, you need to move it back and forth in the glass tube several times to remove all the small amount of cytoplasm."
"The genetically modified pig cells use the same method. After removing the nucleus, they must be injected directly into the oocyte with the nucleus removed within 5 minutes."
"You must move quickly, firmly and accurately. Don't make mistakes or be sloppy."
"It will definitely be a little confusing at first, but once you do it more often, practice makes perfect."
"If the cloning experiment is done well, even if you don't engage in scientific research in the future, it will still be of great use to do in vitro fertilization."
"Of course, even if the country works hard to promote birth, I don't want you to do this all at once. Hahaha~"
"Okay, after the nuclear transfer is completed, put the oocytes in this special solution and let it sit for 1-6 hours."
"When the situation is appropriate, divalent strontium ions (Sr2+) and cytostatin B should be added for treatment."
"The former activates oocytes, and the latter inhibits polar body formation and chromosome elimination."
"The specific method that is appropriate for the situation depends on Brother Wei's experience."
"Let me tell you, if you don't soak it in the laboratory for three to five years, you can't tell it at a glance..."
"After the processing is completed, the oocytes are removed and placed in a special solution without strontium and cytostatin B."
"This is the moment to witness the miracle. The oocyte will gradually divide, form a blastocyst, and slowly turn into an embryo."
Huang Qin presented the entire process completely to the students, and at the same time shared his experience without hesitation.
Under his Chun Chun guidance, within a week, everyone learned how to process pig oocytes.
There were several failures during the period, but fortunately there were enough genetically modified cells in the laboratory, which supported everyone to complete the cloning experiment.
The development of oocytes was also very smooth, and they all developed into embryos at different stages.
Subsequently, embryos at different stages were implanted into sows showing pseudopregnancy for subsequent development.
Embryos that develop earlier, such as those at the cell and blastocyst stages, are implanted into the sow's oviduct and implant naturally.
Those that develop to the blastocyst stage are directly implanted into the uterus, skipping this step.
Each of the students was assigned a sow. After completing a series of treatments, they quietly observed it.
Everyone is waiting for the critical threshold of 20 days to arrive.
Their emotions were very complicated, they were both anxious and uneasy, but also full of confidence.
The smooth progress of the preliminary experiments gave them an illusion.
It seems that this cloning experiment is not too difficult.
As long as you are proficient in the movements, it is not easy to do it well.
As for sows carrying fetuses and giving birth, what's so difficult about that?
Have you never seen a pig running or eaten pork?
Pigs are such a tenacious animal, so there will definitely be no problems.
However, scientific research is not always satisfactory.
"Oops, my baby pig has no fetal movement. What's going on?"
In the laboratory, a female classmate with a high ponytail suddenly let out a scream, and she became panicked.
She jumped onto the cage, looked at the bleeding sow under her body, and let out bursts of exclamations.
However, her cry did not elicit any response from her classmates.
Even if other students heard this, they were shocked and immediately focused all their attention on their sows, how could they possibly care about her affairs.
The tall and lanky boy next to him was staring intently at the sow in the cage in front of him, completely immersed in caring for the sow.
The whole person seemed to be deaf, and the screams from his classmates one meter away did not affect him at all.
Screams seemed to be contagious, and after a while, the fat boy on the other end also let out a shrill scream.
"Ah! My sow had a miscarriage!"
"Your sister, the first time I delivered a baby, I actually gave it to the sow!"
The fat boy's scream became louder and sounded unusually penetrating.
At this moment, his chubby face was flushed red, and his expression was surprised, shocked, and a little bit unbelievable.
The whole person was stunned at the scene, looking at a loss.
However, no matter how unacceptable it was, he had to face the bloody fact.
After reacting, he quickly pulled out an electronic version of "Postpartum Care of Sows" and quickly browsed it on his mobile phone.
"Well, don't panic, find a way to deal with it first."
"Here, after a sow has aborted, it is first necessary to determine whether the stillborn fetus has been completely eliminated."
"If it is not completely discharged, cloprostenol should be injected into the sow first to cause the sow's uterine opening to open and expel the stillborn fetus. Otherwise, the stillborn fetus will rot in the uterus and cause the sow to be infertile."
"After all the fluids are drained, the Guanlin Xianfeng + Immunization Fast Food Injection is injected to reduce inflammation in the sow for 3 consecutive days. This has a very good preventive and treatment effect on the sow's inflammation."
"Huh~~ It's okay, it's okay. Fortunately, I was prepared."
After finding a way to deal with it, the fat boy took a long breath and calmed down.
He followed the methods described in the book and carefully treated the aborted sow, finally saving the sow's precious life.
However, he did not waste the stillborn fetus and quickly took it under a microscope to see what caused the miscarriage.
The same tragedies happened one after another in the laboratory.
The horrific screams came one after another, even reaching outside the laboratory.
On this day, all the teachers and students passing by Du Biao's laboratory were whispering to each other.
"Hey, you passed by Teacher Du's laboratory today. Did you hear any strange noises?"
"Ah, I was just about to tell you. Oh my god, I don't know what happened inside. The cries and howls scared the students upstairs so much that they thought some human tragedy or experimental accident had happened."
"I'll go, I'll be downstairs. The ghosts have been screaming upstairs all day long, making it impossible for me to do any experiments."
"It's not like hitting a ghost."
"Don't talk nonsense, talk about science. I think poisoning is more likely."
"No way, I didn't see the ambulance coming either?"
"Do you think they are infected with swine fever or something?"
"It's possible. In the past few weeks, I have seen people in their laboratory. Everyone is jealous and slouched when walking. They must be sick."
"Everyone, please stay away and don't get infected."
"I took a few sneak peeks through the glass window. The people inside were screaming heart-piercingly. It felt like a walking zombie. Oh my god, it was really scary."
The news spread quickly on campus and soon reached the ears of the hospital.
So, in the distant capital, Du Biao, who had just attended an academic conference, received an urgent call.
"Teacher Du, something happened in your laboratory."
"What? Something happened? What happened?"
Du Biao was confused. He only received a call from a student two days ago, reporting that he was conducting a pig cloning experiment and everything was going well.
"Dean Lu asked you to come back and deal with it quickly. Your students are all in bad condition, and the hospital does not dare to interfere too much."
"Understood." Du Biao heard it. The matter was serious. The students must have gone wrong in the experiment. They were under too much psychological pressure and were on the verge of collapse.
I immediately bought a plane ticket and rushed back after a long journey.
He was well prepared to deal with the aftermath, and also planned to postpone all meetings and provide psychological counseling to each of the students.
However, he never expected that when he pushed open the laboratory door.
What appeared in front of me was such a scene.
(End of chapter)
