Kunming City, Suzhou University, Sanqing Branch.
In a well-lit laboratory with three large windows, Huang Qin's fingers were flying and he was typing on the keyboard quickly.
After watching lines of text flow out of his fingertips, converging into a research report with detailed data, he raised his head and stretched out.
On the long table beside him, there were eight young people with immature faces, who were also busy like him.
Some were thinking hard in front of the computer, some were observing something carefully under the microscope, and others were sitting together in twos and threes, seemingly having an in-depth discussion on the experimental topic.
Huang Qin couldn't help but nodded with satisfaction. Under his influence, the enthusiasm of these juniors and juniors in learning has been visibly improved. Everyone has become diligent and hardworking, which really makes him happy.
Suddenly, a strong cheer erupted from the corner.
A fat boy's face turned red and he jumped up excitedly.
"It passed, it passed, and my paper was accepted."
"I never thought that a poor student like me would one day publish CSCD core journals."
Another tall and thin boy stood nearby and exclaimed.
"The core of cscd, tsk tsk, amazing. Where did you send your paper?"
"Biotechnology Bulletin."
"It must be the article "Establishment of Porcine Six1 and Six2 Gene Knockout Cell Lines", my brother is amazing."
"Haha, blah blah blah, the impact factor is not high, only 0.8." The fat boy scratched his head in embarrassment.
"It's okay. It's our first time to write a paper. It will be good if it can be published." The tall and thin boy showed an understanding smile.
"That's right. I just wrote a random article. I didn't expect it to pass."
"Thanks to the good topics taught by senior brother, otherwise it would be so easy to publish papers."
While the tall and thin boy was talking, his cell phone rang. When he opened it, he saw that it was also a review reply.
He immediately shouted excitedly: "Haha, my paper has also passed. It is "Progress in Biochemistry and Biophysics"."
The fat boy immediately cast an admiring look: "Wow, SCI District 2 paper, senior brother, you are so awesome."
"I want to learn from you and vote for District 2 next time."
The movement of the two people instantly attracted the attention of others.
Everyone put down the business at hand, surrounded the two people, and started talking.
Huang Qin also stretched his neck curiously and looked over there.
He knew in his heart that 80% of the papers everyone had sent out before had received replies one by one.
These papers were all arranged according to his project tasks. His supervisor Du Biao also made minor revisions before submitting them. There will definitely be no problem if they are published in a second area or a core journal.
After all, they are not the top journals in the region. As long as these students' papers are written without flaws, can be self-explanatory, and have solid experimental data, and if they summarize and analyze the rules a little, there is no reason why they should not pass.
Mainly because the direction of pig organ transplantation is very cutting-edge, and many fields are still being explored.
As long as there are some original ideas, richer experimental content, and support from a large number of experimental results, it will be an excellent paper.
Of course, it is impossible for these students to come up with any groundbreaking theories. In his opinion, they are just doing some trivial genome analysis work, and then writing a thesis to complete the graduation requirements.
Thinking of this, Huang Qin couldn't help but clapped his hands and reminded everyone with a smile.
"You guys all look at your mailboxes, maybe there will be a surprise too."
Everyone suddenly realized this and took out their mobile phones to check their emails.
Sure enough, a scream sounded instantly.
A girl with a ponytail waved her mobile phone and was a little incoherent with excitement.
"I passed it, I passed it, and it's also a second district journal."
"Ahhhhh, I'm so excited."
"Now you can graduate directly with a master's degree."
Immediately afterwards, others also cheered.
"I have also passed the core journals of cscd. I will continue to work hard and write more articles."
After reading the email, this person suddenly became very ambitious. He wished he could take similar experimental data and write another article on the spot from a strange angle.
Just for a while, screams came one after another in the laboratory.
Students all receive responses from the journals to which they submit their papers.
Without exception, all papers have passed the review and can be published in major journals.
The ones with the lowest level are all in cscd core journals, with about 5 people.
The top three were all awarded SCI Zone 2.
For a bunch of newbies, this is a dream start.
In the first half of the year, they didn’t even know how to do the experiment.
Even the gene editing technology was learned in the laboratory with Huang Qin after the project began.
The division of labor between Huang Qin and his mentor Du Biao is very clear. The mentor is responsible for preaching about employment and answering questions, and attends meetings everywhere to make them familiar.
He was responsible for teaching the younger brothers and sisters to do experiments, assigning tasks to them, and watching them work.
Of course, this kind of paper correction must be done by the instructor. After all, he is the only one who has rich experience in publishing papers in SCI District 2.
Huang Qin only has experience in papers published in top publications in the first area.
The standards of the two are different, and the difficulty is also very different.
Of course, there is no need to use a sledgehammer to kill a chicken, so there is no need for him to revise the papers for these cute little guys.
He just needs to do a good job as a contractor while completing his own tasks.
These students are very well-behaved, much more obedient than the employees in his department. They dare not go east when told to go west, and they are as docile as little sheep.
Every time he taught these rookies to do experiments, Huang Qin felt like he was reliving yesterday.
It felt like I was back on my first day working at Sanqing, looking at a lot of instruments in front of me in the laboratory, with a blank expression and a loss.
Fortunately, Dawei led him to get started slowly, and he went through simple experiments one after another, and he was where he is today.
Sometimes I think about how happy it would be for a newbie to have a guide who can teach him step by step when he first learns to do experiments.
Perhaps because he had been caught in the rain and had an enthusiastic umbrella holder, he did not hesitate to be the next umbrella holder.
Anyway, when teaching these junior brothers and sisters to do experiments, he took the trouble to teach them all the experiences he learned at work, starting from the smallest details.
He has been doing experiments in Sanqing for so many years, and he has become very familiar with it. He knows the simplest and most effective experimental methods, as well as all the precautions.
For him, teaching his years of experience to these students is also a kind of enjoyment of being a teacher, and it is also a journey of practice that summarizes experience into rules.
In the first two months, the students still had some problems, but with the incentive of project bonuses and graduation thesis, they still studied like a chicken blood, forgetting to eat and sleep.
With ample funding, they were able to repeat experiments and make progress.
With the encouragement of their instructors and Huang Qin, the students' confidence greatly increased and they felt like a duck in water.
They obtained experimental results from the laboratory's separatory funnels, centrifuges, colorimeters, and PCR sequencing machines, gained unprecedented scientific research experience, and were promoted to a noble, rational, and noble academic kingdom.
In just three months, Huang Qin has already seen that there are several good candidates suitable for scientific research.
And all of this finally bore fruit today.
Within a short period of time, everyone got results.
Huang Qin also saw their paper list on the computer.
"Establishment of Pax2 knockout pig cell line"
"Analysis of molecular information of porcine SALL1 protein and preparation of gene knockout cell lines"
"SCD gene knockout porcine cell line and its construction method"
"Construction of porcine vascular endothelial cell surface antigen gene knockout cell line"
"Construction method of a genetically modified cell surface Fas molecular pig model"
"Comparison of Homology of Human/Pig Complement Regulatory Proteins and Establishment of Porcine GGTA1 Gene Transferred Cell Line"
"Establishment of porcine Six1 and Six2 gene knockout cell lines"
"Establishment of porcine cell lines transformed with humanized TFPI coagulation regulatory gene"
The eight papers are all related to pig research.
Either insert foreign genes into the pig cell genome, or knock out the relevant endogenous genes.
In this way, knocking out specific antigen genes and transferring into humanized genes can not only effectively regulate immunologically incompatible genes, but also improve coagulation dysfunction, thereby effectively inhibiting the occurrence of transplant rejection.
However, with only eight papers, this is far from enough.
These immature students can still squeeze more results out of them.
The cleaner it is squeezed, the richer the harvest will be.
There are more than 30 immune and coagulation genes in total. Only by improving all genes can we understand the relationship between them and find out the most critical genes.
These studies can produce at least 20 more papers. Evenly divided, everyone has three or four papers, which is enough for them to be busy.
Not only must people be squeezed clean, but also the most oil and water must be squeezed out of the pigs.
Thinking of this, Huang Qin's eyes suddenly lit up and he said with a smile.
"It seems that everyone has gained a lot from the research over the past few months."
“It’s really gratifying that no one has missed out on the 8 papers.”
"I don't know if you still remember something I once said."
"What are you talking about?" Everyone immediately fell silent, their ears perked up, and they all looked over.
"I have told you a long time ago that for every paper you publish, you will receive a bonus of 20,000 yuan. Have you all forgotten? This shouldn't be the case."
Huang Qin burst out laughing, looking incredible.
Students, you looked at me and I looked at you, and immediately started laughing too.
"Senior Brother, how could you forget this? I'm just waiting for you to say it yourself." The fat boy joked.
"You are our bread and butter. Everyone calls you Senior Brother respectfully. No one dares to rush you."
Huang Qin chuckled: "I bet this is still my fault. Come on, hurry up and go through the process and submit the application to me. If you hurry up, the money should be in your account by the end of this month."
Everyone was overjoyed immediately, and everyone smiled so brightly that their teeth could not see their eyes.
"Wow, it's really great. I'll be rich at the end of the month."
When everyone thought that in addition to the 5,000 yuan living allowance, there was also a 20,000 yuan paper bonus, and they could get 25,000 yuan at the end of this month, they suddenly felt like pie in the sky.
I wanted to pinch my thigh immediately to see if I was dreaming.
Huang Qin looked at this scene and was very satisfied and asked with a smile.
"Isn't it great to publish a second-district paper?"
"It's cool!" Everyone couldn't help but nodded.
"Wouldn't it be great to receive 20,000 yuan after publishing the paper?" Huang Qin followed suit.
"It's great, it's so great!" Everyone shouted loudly.
"Then do you want to publish more papers and earn a few more 20,000 yuan?" Huang Qin finally came up with the idea.
Everyone said in unison: "Of course I do."
"Elder brother, don't love me just because I'm a delicate flower, and torture me severely with money."
"Don't stop!"
"There is a lot of money and a lot of papers. Who wouldn't want such a good life?"
"That's right. If you have any experiments, just come over and come to me!"
Everyone had published their papers, had money in their pockets, and suddenly felt confident.
Huang Qin couldn't help showing a proud smile like an old fox: "I'm just waiting for you."
He swiped a few times and sent a list of experiments to everyone.
"There are more than 30 immune-related pig genes that we need to study. We have already done some of them. I have discussed other experiments with my tutor and they are in this list."
"You pick slowly one by one and find the ones you are interested in. At least 3-4 each person should mark them. We will start doing new experiments tomorrow."
"That's almost it. Everyone shouted so enthusiastically just now, I hope you won't disappoint me."
Everyone couldn't wait to open the file and took a breath of cold air when they saw it.
"Hiss, there are so many?"
"I never thought that a pig could water so many papers!"
"Holy shit, how long have you been preparing for this, Senior Brother?"
"If we hadn't had so many people, I might have had to wait until the end of the year."
"This time it's not just 996, it's 007."
"Although there are many experiments, there is also a lot of money. If I choose 4 experiments, I can publish 4 papers. Plus the one I just published, my dear, the bonus alone will be 100,000 yuan."
Now, everyone was not calm.
Soon, within five minutes, everyone was enthusiastically sharing the experiment.
Everyone was assigned 4 experiments, and none of them failed.
Huang Qin waved his hand with great satisfaction: "Students, next, it's time to get excited."
"Don't worry, I will definitely stand with you and accompany you to have fun together."
"From today on, I will eat with you, live with you, and work with you."
"We must understand the functions of all immune-related genes as soon as possible, and then create the cleanest pig blastocyst. After it matures, its organs can be transplanted to baboons for further clinical verification."
"If the feasibility of gene knockout pig-baboon xenotransplantation is confirmed, it will be the biggest breakthrough in the field of organ transplantation in recent years."
"Foreign scientists have conducted similar experiments, transplanting unmodified pig livers to non-human primates. As a result, xenoimmune rejection occurred immediately after the transplantation, and the survival time did not exceed 12 hours."
"Later, scientists transplanted GGTA1 knockout pig livers into baboons, but they only survived for 5 days."
"Although gene knockout prolonged the survival time of transplanted organs, other immune rejection reactions persisted, as well as more severe coagulation disorders."
"So, we have to get this out of the way and continue research on this."
"Only if the xenotransplanted organ can survive healthily in the baboon's body for more than three months without complications can we prove our success."
"I'm very much looking forward to waiting for this day to come with you."
