There were two circles of people in the laboratory, one on the left and one on the right, one making noise and the other quiet.
People in the circle on the right are sequencing in silence, with serious attitudes and methodical movements.
Here are two new sequencers made by Daxia Intelligent Technology. Although their working principles are different from those of Illumina, they are both second-generation sequencing technologies and everyone is familiar with them, so it is not too difficult to operate them.
They are now using the new machine to test the samples from the gene bank, familiarizing themselves with the operation of the platform, analyzing the data, and then comparing the data from different platforms.
Second-generation sequencers take longer to produce results, taking several days. They have done the test and just need to wait for the results.
The circle on the right was very lively, with people forming a circle and talking a lot.
There are three small and exquisite, palm-sized sequencers placed on the operating table inside, and three employees are operating them carefully.
Standing next to the three people was none other than the gentle Jiang Kai, who was also promoted and became the director of the genetic laboratory.
At this moment, he was smiling broadly, staring at the three instruments with bright eyes, and was assigning tasks.
"New toys are here today. Don't be anxious. Come one by one, and everyone will have a chance. You can first select the gene sequence you want to test, and then register there."
"Everyone knows that nanopore sequencers have the characteristics of small size, fast library construction, and real-time data generation. Compared with existing sequencing technology, one of its key advantages is longer read length, which has significant advantages in human genome sequencing. The advantage is that it is easier to detect larger structural changes in a single genome."
"The more fun something is, the more we have to stay behind, from easy to difficult. So today we will start testing bacteria and viruses, then start testing mice, and finally test human DNA sequences."
"Someone is already testing E. coli, yeast, and adenovirus. You have to think of some new species next, and you cannot repeat the test."
"After everyone takes the test, they need to analyze the data, compare it with known data, and submit a report."
The crowd cheered and everyone was eager to try.
Huang Qin took a look at the sequencing targets of other colleagues. They were diverse and included everything from bacteria and viruses to animal and human DNA.
He registered his DNA. He was very curious about the ability of nanopore technology to test long-sequence genomes. His own genome had been tested and he was familiar with the data, so it was naturally the best control sample.
Williams made the same choice.
After Huang Qin registered, he squeezed next to his colleagues who were working and watched carefully.
The colleague carefully put the E. coli test kit into the machine, connected it to his laptop, and started waiting for the real-time results.
At the heart of the machine, an enzyme unwinds the double strands of DNA and threads one strand through a protein pore. The unique shape of each DNA base causes unique electrical interference to read out the sequence.
In the whole process, the long DNA chain is like a long queue of people at the subway ticket gate. Each base is like a passenger, entering a narrow nanopore. There is a swipe inside, the current flashes, and the identity is verified. The next person continues through.
Soon, the real-time reading data of the DNA fragments was displayed on the computer software.
The operator exclaimed while watching: "It's incredible. It's really fast and accurate. It matches the previous test data by 98%. Although the accuracy is still a little bit behind, there is still no problem in actual use." "
Jiang Kai quickly stepped forward, leaned in front of the computer screen, quickly scanned it, and nodded approvingly.
"This thing is really good. In the future, we can sequence and test as much as we want without having to wait until a certain number is collected before testing."
"The E. coli you tested is a drug-resistant bacteria, right? Look here, its genome contains a large number of structural variations. These cannot be detected using the data of the second-generation sequencer. It seems obvious now that it is very important to us. Studying its resistance mechanisms is very helpful."
"This detection technology can realize real-time output of data and real-time analysis, which is really suitable for rapid detection of microorganisms."
Suddenly, warm applause sounded in the laboratory, accompanied by bursts of whispers.
"This is an improvement in domestic technology!"
“I didn’t expect domestic sequencers to be so advanced.”
“I’ve only heard of it being available abroad, but I didn’t expect it to be made in China now.”
"Looking at it like this, it's no worse than foreign equipment."
"It's so convenient. I no longer have to wait several days for results."
Everyone had excited expressions on their faces, and they were still discussing endlessly until they returned to the office.
Huang Qin was also shocked. He looked at the dumbfounded Williams beside him, and felt an inexplicable joy in his heart.
"Dawei, have you ever used a nanopore sequencer before? Is there a big difference between it and this machine?"
Williams looked shocked and could not help but burst out with exclamations: "Amazing, unbelievable, this is a machine made in China. I have used a machine from Oxford Nanopore before, and that was several years ago. , the machine is still very crude, incomparable to this one."
"But their new machine is very mini, only about the size of a chocolate bar, and its performance has been greatly improved. It should be comparable to this one."
Huang Qin nodded reservedly and looked at the sequencer made by Daxia Intelligent Technology: "With the fourth-generation sequencing technology, it seems that we can overtake it. I just don't know if the second-generation machine can match Illumina."
"Yes," Williams also looked over, a little worried: "If the second-generation sequencer is no different from Illumina, then Illumina will be in danger. There will be a big change in the global sequencing field. "
Three days later, the sequencing results from Daxia Intelligent Manufacturing also came out.
Jiang Kai looked at the analysis data in his hand, frowned, and then relaxed again.
"Compared with Illumina, there is no difference in reading length among mainstream dosage forms, they are all fragments of 50-150."
"There are some differences in the quality of the data obtained. Inmine's is more accurate and can reach 99.9%. Daxia Intelligent Manufacturing's is about 97~99%. The data is unstable and may need to be tested several times. But this is originally a splicing gene. Fragment, it should be technically solvable.”
"Generally speaking, the accuracy of the test data is not bad, but if the algorithm can be optimized, the accuracy will be higher."
"The data analysis is a bit awkward, and it is far behind Illumina. It is not as easy to use as our own software."
"The test results are usable, but we need to optimize the algorithm and use our own data analysis software."
The analysis results were reported quickly and received positive approval.
****
In the dead of night, Williams tossed and turned, unable to fall asleep for a long time.
Finally, he stood up and took out an old laptop from the suitcase under the bed.
After some confidentiality, an email was sent to an address that looked like garbled characters.
"That's a bad move."
"After the entire Sanqing line is localized, we will suffer the loss."
Five minutes later, there was a reply.
"Mind yourself."
"Don't teach me how to do things. Sanqing is just a special case, there will be no second one."
“It’s not that easy to make all products local.”
"Overall, the advantage is in my favor."
The corner of Williams' mouth twitched, and he was suddenly speechless, with an unknown fire rising in his heart.
So arrogant!
Gentlemen on the other side of the ocean have been in the office for a long time, and the space they live in has become folded with reality.
They couldn't see Sanqing's true strength, unlike him, who was among them and knew exactly how terrifying Sanqing was.
That's all, he's just a worker.
Why bother with so much, just do your own thing.
After all, that's what it was directed from above.
