In the classroom of the Academy Supervisor Building, Yang Yu raised his hand and asked, "Doctor, why didn't Li Ang come to class?"
"He's conducting very important research."
Chao Wenyuan, a doctor of mathematics, said with a straight face: "Not only for today's class, you won't be able to see him for some time in the future."
"ah?"
Yang Yu and others looked stunned and asked subconsciously: "What kind of research takes so long? How could Jijiu agree to approve a fake that takes so long?"
"It's not arithmetic anyway!"
Chao Wenyuan patted the table, blew his beard and stared, muttering something in a low voice, "It would be a waste of resources for such a good young man not to study arithmetic..."
————
Outside the Xuegong Patent Office, Hu merchants and Jing merchants wearing gorgeous silk costumes were holding stacks of flying money banknotes, surrounding Su Feng, Ph.D. of Science.
"Dr. Su Feng, where is Mr. Li Xiaolang? Didn't I say that we can see him in a few days?"
"Yes, Dr. Su Feng, we have all the money ready. We are just waiting for his permission to transport the goods back."
"Doctor, our caravan is eating horse chews in Chang'an City, which costs money every day, and we really can't afford to wait. Please go ask Mr. Li Xiaolang, please."
The huge stacks of flying money waved by the businessmen made the apprentices in the patent office dizzy.
Su Feng had no choice but to release his monk momentum and push back the crowd, "I'm really sorry, I can't help with this. Li Ang is very busy during this time and doesn't see anyone else. You should go home and wait for notification."
————
In the north of Chang'an City, there is Daming Palace and Hanxiang Hall.
"That little doctor Li didn't come? Didn't you tell him that this was for the sake of the emperor's heir?"
Concubine Zheng, who was born in Qiwang of the fifth surname, was sitting on a bench, caressing her ten-month-old belly, and scolding the chamberlain who was kneeling in the palace.
"Sister, don't be angry, it's not good for the emperor in your belly."
Another Concubine Lu, who was also born in the fifth surname and Qiwang, gave a soft word of comfort, looked at the chamberlain and said: "You go to the academy again and say that the imperial doctor said that the delivery date will be in the next few days. Please invite Li Xiao Every day after the doctor returns home from school, he comes to Daming Palace to take a look.
No need to delay anything,
When the emperor's heir is born safely, the Zheng family will remember his kindness..."
As the concubine surnamed Lu was speaking, she suddenly noticed the fearful expression on the eunuch's face. She paused and said hesitantly: "You didn't see that little doctor Li, or did the academy simply not let you in? Who stopped you? ?”
"Yes...it's sacrificial wine."
The eunuch lowered his head and said in a trembling voice: "The sacrificial wine asked me to bring a message to the two noble concubines."
The two noble concubines stood up together, "What are you talking about?"
"Everyone, calm down."
————
In the afternoon, Chai Cuiqiao, who was picked up by the academy carriage at the foot of the back mountain, jumped off the carriage carrying large and small packages.
"Master."
Chai Cuiqiao, who was confused, glanced at the carriage that was driving away quietly, turned to look at Li Ang who was standing at the intersection, and said doubtfully: "What are we..."
"We will be living in the academy for the next few days."
Li Ang smiled apologetically, took the package from Chai Cuiqiao's hand, and took her to an unknown pavilion at the foot of the back mountain.
There is a dazzling array of experimental equipment on the first floor of the pavilion, and the bedrooms are on the second floor.
The Academy attaches great importance to blood carbuncle medicines.
After receiving the news, Jijiu applied to the mountain chief and provided Li Ang with an entire pavilion as an experimental site. He also granted him a long vacation at once, and he would not lose credits if he failed to take courses such as Yu Lv and National History.
"What's this?"
Chai Cuiqiao put down her change of clothes and toiletries and curiously poked the flask on the table.
"This is the distillation flask, this is the condenser tube, this is the vacuum connecting tube, this is the receiving flask, this is the capillary tube..."
Li Ang introduced the experimental instruments to Chai Cuiqiao in detail. These things were made of hot glass by the monks of the Academy using their telekinesis.
"What about this?"
Chai Cuiqiao pointed to a huge metal device with a cylindrical structure next to the wooden table.
"Simple circulating water vacuum pump."
Li Ang said with a smile: "The impeller rotor is installed eccentrically in the cylindrical pump casing, and the blades of the rotor are tilted forward.
The pump body contains an appropriate amount of water as the working fluid. When the impeller rotor rotates, the working water will form a water ring that swirls along the pump casing under centrifugal action.
There is a certain space between the closed rotating water ring and the impeller rotor. When it rotates over and over again, the air can be pumped out.
Due to the poor conditions, I could only ask the academic doctors to design it in the form of human power rotation.
The faster it spins, the more efficiently it pumps air. "
"Well...."
Chai Chai blinked, "What does this...pump do?"
"Reduce the pressure or even create a vacuum."
Li Ang waved his hand and said casually: "Of course, in this experiment, its main function is to perform vacuum distillation.
And the distilled stuff..."
Li Ang picked up a large bag of things from under the table and threw it on the table.
"This is..."
Chai Chai said in shock: "Garlic?"
"That's right."
Li Ang smiled and said: "The antibacterial ingredient that can solve blood carbuncle, or infection and inflammation, is in garlic."
Diallyl thiosulfinate, or allicin, is an organosulfur compound.
It is not an antibiotic, but a broad-spectrum antibacterial drug.
It has inhibitory and killing effects on a variety of cocci, Bacillus pertussis, Bacillus diphtheriae, Bacillus dysentery, Bacillus typhoid and paratyphoid, Mycobacterium tuberculosis, etc.
It can even deal with a variety of fungi, viruses, and even amoeba that can cause meningoencephalitis, keratitis, and pinworms that parasitize in the intestines.
Allicin can be used internally or externally. It has few side effects and is not likely to cause the corresponding fungi or bacteria to develop resistance - because its bactericidal mechanism mainly directly destroys enzymes containing thiol groups.
It is extremely difficult for fungi and bacteria to mutate and crack.
The most common pyogenic cocci, Staphylococcus aureus, happen to be within the killing range of allicin.
Considering that the preparation of penicillin, another spectrum antibiotic, is relatively difficult, allicin can almost be said to be the most easily available antibacterial drug at this stage.
The only problem is how to make it.
There is no allicin in fresh garlic, only alliin. When garlic is cut or crushed, alliinase is activated and catalyzes the decomposition of alliin into allicin.
To eat raw garlic, according to the effective dose, you have to eat at least several kilograms or more than ten kilograms at a time.
It must be purified.
In the next few days, Li Ang and Chai Cuiqiao stayed in the pavilion, busy using various methods to extract allicin.
After experiments, there are two most feasible methods of extracting allicin at this stage.
1. Steam distillation method under reduced pressure.
First peel and wash the garlic, mash it in a stone mortar, pour it into a glass container, pour in distilled water and cover it with a lid.
Use the salt bath method to heat it slightly to enzymatically hydrolyze it.
Then use a circulating water vacuum pump to perform vacuum distillation of water vapor.
After collecting the distillate, distill under reduced pressure again.
Cycle several times to obtain the solution and store it in a simple refrigerator made of ice trays - allicin is extremely unstable and loses its antibacterial activity when exposed to heat or alkali. Its validity period is very short at room temperature.
Coupled with the strong and touching taste and other reasons, it cannot compete with other antibacterial drugs in the memory of another world.
2. Ethanol extraction method.
Peel the garlic, wash it, mash it, enzymatically hydrolyze it with low heat, pour ethanol of different concentrations, use a reflux tower to fully dissolve the allicin in the ethanol, wait for several hours, and rotate the garlic pulp at more than a thousand revolutions per minute. Centrifuge at high speed, filter through double-layer rice paper, and discard the garlic residue.
The filtrate was distilled under reduced pressure and repeated several times to obtain a solution.
The entire extraction process lasted several days,
The exhausted master and servant looked at the hundreds of test tubes containing light yellow oily liquid allicin on the table, and finally smiled.
It's so stinky, the process of extracting allicin is just so stinky.
It was like a gastronomic symposium between canned herring, stinky tofu, and kiwiak (the Eskimo food made from rotten petrel seals).
Even if the windows are fully opened and the breeze charm is used for ventilation at all times, the master and the servant feel that they can't stand it.
"Master, I stink so much."
Chai Chai smelled his clothes and said with a sad face.
"It's okay, I stink too."
Li Ang comforted him and asked Chai Chai to go back to Jinchengfang to wash up and rest, while he walked out of the pavilion carrying a box of test tubes.
