Chapter 78 Dawn

"In agriculture, a paper is just a summary of your work. The essence of evaluating the quality of your work is the positive impact your research results have on agriculture. Publishing a paper is only an essential side effect."

"I have no way to convince you to believe my method now. The maturity period of cabbage is about forty days. If you take good care of the crops carefully, the final results will prove that I am right."

Dong Wenxu didn't know where Jiang Yan's confidence came from. He just hoped that his work during this period was not in vain.

However, during the planting process, Dong Wenxu could enjoy it. He sometimes thought that the Chinese people's farming genes were too strong.

Farming is an instinct deeply rooted in the genes of Chinese people. After Jiang Yan obtained the standard magic brain, he continued to invest in agricultural research.

Jiang Yan can sense life, so Simmons also has this ability, and the characteristics given by the standard magic brain are the same.

Simmons joined Pfizer's special drug research and development team, and Amerikan hoped that he could work its magic.

The researchers identified twelve proteins that were identified as potential targets.

They can be roughly divided into two categories: the first is structural proteins, including spike proteins, small envelope proteins, membrane proteins, nucleocapsid proteins and another category of proteins called non-structural proteins.

Among them, non-structural proteins mainly include protease (M pro), papain, nsp13: helicase, nsp12: RNA-dependent RNA polymerase, nsp14: N-terminal exoribonuclease and C-terminal guanine-N7 methyltransferase, nsp15: urine Nucleotide-specific endoribonuclease, nsp16:2'-O-methyltransferase, and nsp10.

The virus is responsible for the replication and packaging of viral nuclear material into the capsid protein shell.

Blocking any of these proteins causes a stall in the virus's replication or packaging of its RNA into a protein coat.

Among a total of 12 proteins identified as targets to control CoV; 3CL pro was identified as one of the most important targets due to its critical role in mediating viral replication and transcription.

3CL pro is a chymotrypsin-like cysteine ​​protease (~33 kDa) that is similarly known as the major protease due to its dominant role in processing replicase polyproteins and genes.

Although 3CL pro is a dimer, which provides the substrate binding cleft, the protease exerts its proteolytic activity through nucleophilic attack directed by cysteine ​​thiols in its Cys-His bimolecule.

Evidence of the activity of 3CL pro during the viral life cycle is lacking from its human analogues.

Therefore, Pfizer researchers only consider 3CL pro as a potential primary drug target to bind and inhibit viral replication activity.

They need Simmons to judge from his perspective.

There is a high degree of similarity between the 3CL pro structure and 3CL pro of different virus mutants, and inhibitors previously designed for MERS or SARS can also be effective against the currently prevalent viruses.

At the same time, an inhibitor called N2, previously designed to inhibit 3CLSARS-CoV and MERS-CoV, was also tested against the virus this time.

Some researchers have proposed targeting 3CL pro, among which the efficacy of available drugs such as ritonavir, emetin, hesperidin, lopinavir, and indinavir have shown potential to inhibit 3CL pro.

The phytochemicals of green tea have also been evaluated in depth to identify phytochemicals with the potential to inhibit 3CL pro.

In addition, the phytochemicals of neem were also evaluated against the target proteins of SARS-CoV-2, namely membrane protein (M) and envelope protein (E), among which Nimocin, 24-Methylenecycloartan-3-one, phytosterols, Beta-Amyrin and Nimbolin A were injected into mice by Pfizer researchers.

Simmons touched the mice one by one. I don’t know if it was his psychology or it was really the case. He thought that touching the mice with his own hands would help him make a better judgment.

"I think this is just the best guinea pig out there."

The researchers next to him quickly disinfected him, and another researcher asked: "This mouse was injected with a protease inhibitor."

"We developed protease inhibitors based on five popular M pro variants in the new coronavirus lineage, including G15S, T21I, L89F, K90R and L205V."

"I think we need more observation. We have just completed the preliminary injection. It is still unknown whether the mice injected with protease inhibitors can still maintain their best condition."